Fluence Compensation algorithm validation. (A) Schematic of a phantom with 3 layers of gelatin phantom mixed with intralipid/ink solution and 3 carbon lead imaging targets, (B) photograph of the phantom made in a cube box and magnified to show the layers, (C) Ultrasound (US) image of the phantom, (D) Photoacoustic (PA) image before fluence compensation, (E) Monte Carlo simulation of light propagation in the phantom, (F) PA image after fluence compensation.

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